Bicyclic octahydrocyclohepta[ b ]pyrrol-4(1 H )one derivatives as novel selective anti-hepatitis C virus agents

• DOI: 10.1016/j.ejmech.2016.06.041
• Publication Date: 2016-06-25T19:20:02Z
• Authors: Neerja Kaushik-Basu; Nina K. Ratmanova; Dinesh Manvar; Dmitry S. Belov; Ozge Cevik; Amartya Basu; Mark M. Yerukhimo...; Evgeny R. Lukyanenko; Ivan A. Andreev; Grigory M. Belov; Giuseppe Manfroni; Violetta Cecchetti; David N. Frick; Alexander V. Kurkin; Andrea Altieri; Maria Letizia Bar...
• Keywords: NS5B

We report the discovery of the bicyclic octahydrocyclohepta[b]pyrrol-4(1H)-one scaffold as a new chemotype with anti-HCV activity on genotype 1b and 2a subgenomic replicons. The most potent compound 34 displayed EC50 values of 1.8 μM and 4.5 μM in genotype 1b and 2a, respectively, coupled with the absence of any antimetabolic effect (gt 1b SI = 112.4; gt 2a SI = 44.2) in a cell-based assay. Compound 34 did not target HCV NS5B, IRES, NS3 helicase, or selected host factors, and thus future work will involve the unique mechanism of action of these new antiviral compounds.