The design of a cell-selective fowlicidin-1-derived peptide with both antimicrobial and anti-inflammatory activities

• DOI: 10.1016/j.ejmech.2019.111623
• Publication Date: 2019-08-13T15:54:30Z
• Authors: Ganesan Rajasekaran; S. Dinesh Kumar; Sungtae Yang; Song Yub Shin
• Keywords: 0301 basic medicine; Erythrocytes; Sheep; Dose-Response Relationship, Drug; Molecular Structure; Cell Survival; Anti-Inflammatory Agents; Microbial Sensitivity Tests; Gram-Positive Bacteria; Peptide Fragments; Anti-Bacterial Agents; 3. Good health; Mice; Structure-Activity Relationship; 03 medical and health sciences; RAW 264.7 Cells; Cathelicidins; Drug Design; Gram-Negative Bacteria; Animals; Chickens

Fowlicidin-1 (Fowl-1), a cathelicidin expressed in chicken intestine, is known to have both antimicrobial and anti-inflammatory properties. However, its pharmaceutical development has been ultimately compromised by its high host cytotoxicity. In this study, a series of N- and C-terminal-truncated 19-meric Fowl-1 peptides were synthesized. Among these truncated peptides, Fowl-1 (8-26) exhibited broad-spectrum antimicrobial activity without human erythrocyte cytotoxicity while reducing anti-inflammatory activity. Further, Fowl-1 (8-26)-WRK was designed via Thr5→Trp, Ile7→Arg, and Asn11→Lys substitutions in Fowl-1 (8-26) to exhibit more amphipathicity. The results revealed that it exhibited both antimicrobial and anti-inflammatory properties. This study also demonstrated that the inhibitory activity of Fowl-1 (8-26)-WRK against LPS-induced inflammation was mainly due to the binding of LPS to the peptide. Interestingly, compared with human cathelicidin LL-37 and melittin, Fowl-1 (8-26)-WRK showed more potent activity against drug-resistant bacteria. It was also resistant to physiological salts and human serum and acted synergistically in combination with conventional antibiotics, such as chloramphenicol, ciprofloxacin, and oxacillin, suggesting that combined with conventional antibiotics, it is a promising adjuvant. Furthermore, membrane depolarization, SYTOX Green uptake, and flow cytometry revealed that it kills bacteria by damaging their membrane integrity. Therefore, this study suggests that Fowl-1 (8-26)-WRK has considerable potential for future development as an antimicrobial and anti-inflammatory agent for treating antibiotic-resistant infections.