Enhanced acute anti-inflammatory effects of CORM-2-loaded nanoparticles via sustained carbon monoxide delivery

• DOI: 10.1016/j.ejpb.2016.09.008
• Publication Date: 2016-09-13T15:16:10Z
• Authors: Omer Salman Qureshi; Alam Zeb; Muhammad Akram; Myung-Sic Kim; Jong-Ho Kang; Hoo-Seong Kim; Arshad Majid; Inbo Han; Sun-Young Chang; Ok-Nam Bae; Jin-Ki Kim
• Keywords: Inflammation; Lipopolysaccharides; Male; 0301 basic medicine; Carbon Monoxide; Drug Carriers; Cell Survival; Myoglobin; Macrophages; Anti-Inflammatory Agents; Nitric Oxide; Lipids; 12. Responsible consumption; Mice; 03 medical and health sciences; Drug Delivery Systems; Microscopy, Electron, Transmission; Organometallic Compounds; Pressure; Animals; Edema; Nanoparticles; Particle Size

The aim of this study was to enhance the anti-inflammatory effects of carbon monoxide (CO) via sustained release of CO from carbon monoxide-releasing molecule-2-loaded lipid nanoparticles (CORM-2-NPs). CORM-2-NPs were prepared by hot high pressure homogenization method using trilaurin as a solid lipid core and Tween 20/Span 20/Myrj S40 as surfactant mixture. The physicochemical properties of CORM-2-NPs were characterized and CO release from CORM-2-NPs was assessed by myoglobin assay. In vitro anti-inflammatory effects were evaluated by nitric oxide assay in lipopolysaccharide-stimulated RAW 264.7 macrophages. In vivo anti-inflammatory activity was investigated by measuring paw volumes and histological examination in carrageenan-induced rat paw edema. Spherical CORM-2-NPs were around 100nm with narrow particle size distribution. The sustained CO release from CORM-2-NPs was observed and the half-life of CO release increased up to 10 times compared with CORM-2 solution. CORM-2-NPs showed enhanced in vitro anti-inflammatory effects by inhibition of nitric oxide production. Edema volume in rat paw was significantly reduced after treatment with CORM-2-NPs. Taken together, CORM-2-NPs have a great potential for CO therapeutics against inflammation via sustained release of CO.