Early growth response 1/Krüppel-like factor 5 pathway inhibitor alleviates lipopolysaccharide-induced lung injury by promoting autophagy

• DOI: 10.1016/j.ejphar.2023.176294
• Publication Date: 2023-12-28T02:50:00Z
• Authors: Kang Zou; Cheng Wang; Chaoqi Zhou; Yuting Yang; Zhenguo Zeng
• Keywords: EGR1; Mediator

Early transcription factors play critical roles in the development of acute lung injury/acute respiratory distress syndrome (ALI/ARDS). growth response 1 (EGR1) is a factor essential for various biological processes, including regulation metabolism, differentiation, and inflammation. However, its role ALI has been poorly reported. In this study, we aimed to determine effect EGR1 on gain insights into theoretical basis further treatment ALI. By employing concerted molecular biology techniques, showed that protein was upregulated mice. mice human epithelial cells lipopolysaccharide (LPS) stimulation. knockdown promoted autophagy reduced LPS-induced pro-inflammatory mediator production. preferentially bound GCGTGGGCG motif region EGR1-binding peak-related genes were mainly enriched injury stress-related pathways. Additionally, Krüppel-like 5 (KLF5) by binding KLF5 promoter region, significantly decreased inflammatory damage, suggesting promotes progression regulating expression. Furthermore, ML264, an inhibitor EGR1/KLF5 pathway axis, displayed protective reduce conclusion, our findings demonstrate potential inhibit promote autophagy, reducing mitigate ALI/ARDS. The axis may be valuable therapeutic target