Antiproliferative activity of two copper (II) complexes on colorectal cancer cell models: Impact on ROS production, apoptosis induction and NF-κB inhibition

• DOI: 10.1016/j.ejps.2021.106092
• Publication Date: 2021-12-05T21:52:33Z
• Authors: Maria C. Ruiz; Karen Perelmulter; Pedro Levín; Adolfo I.B. Romo; Luis Lemus; Mariela Bollati -...; Ignacio E. León; Ana Laura Di Virg...
• Keywords: Viability assay

The main goal of this work was to screen the antiproliferative activity and mechanism actions two copper complexes: [Cu(dmp)2(CH3CN)]2+ (1) [Cu(phen)2(CH3CN)]2+ (2) on 2D 3D colorectal cancer cells models. Cell viability studies three cell lines (HT-29, LS174T, Caco-2) displayed that 1 showed more robust than 2 cisplatin. Intracellular content (63.24% 48.06% for 2, respectively) can explain differences in cytotoxicity assay. ROS production is primary action involved showing 4-, 70- 2.5- fold increased values level HT-29, Caco-2 lines, respectively. This effect takes place along with depolarization mitochondrial membrane at µM. Besides, both complexes apoptosis low micromolar concentrations (0.5-2.5 μM). Moreover, inhibited NF-κB pathway HT-29-NF-kB-hrGFP monolayer (0.5 μM) spheroids HT-29 GFP (5 10 inhibitory leads an inactivation MMP-9 expression line. Altogether, these results exhibits human model.