Inflammatory Gene Expression of Human Perivascular Adipose Tissue in Abdominal Aortic Aneurysms

• DOI: 10.1016/j.ejvs.2021.02.034
• Publication Date: 2021-04-13T22:45:57Z
• Authors: Jorn P. Meekel; Marina Dias-Neto; Natalija Bogunovic; Gloria Conceição; Claudia Sousa-Mendes; Gawin R. Stoll; Adelino Leite-Mor...; Jennifer Huynh; Dimitra Micha; Etto C. Eringa; Ron Balm; Jan D. Blankensteijn; Kak K. Yeung
• Keywords: MMP9; Abdominal aorta

ObjectivePerivascular adipose tissue (PVAT) contributes to vascular homeostasis and is increasingly linked pathology. PVAT density volume were associated with abdominal aortic aneurysm (AAA) presence dimensions on imaging. However, mechanisms underlying the role of in AAA have not been clarified. This study aimed explore differences from using gene expression functional tests.MethodsHuman control subcutaneous collected during open surgery. Gene analyses tests performed. The group consisted healthy aorta non-living renal transplant donors. performed genes potentially involved various inflammatory processes related genes. Live (SAT) used for ex vivo co-culture smooth muscle cells (SMCs) retrieved non-pathological aortas.ResultsAdipose was harvested 27 patients (n [gene expression] = 22, n [functional tests] 5) five patients. An increased PTPRC (p .008), CXCL8 .033), LCK .003), CCL5 .004) an increase extracellular matrix breakdown marker MMP9 .016) found compared controls. Also, there a decreased anti-inflammatory PPARG controls .040). SMC co-cultures aortas showed .033) SMTN .008) SAT these SMC.ConclusionThe data revealed that shows pro-inflammatory metallopeptidase expression. Furthermore, formation Therefore, might contribute inflammation adjacent wall thereby plays possible pathophysiology. These proposed pathways induction could reveal new therapeutic targets treatment. Perivascular tests. Human aortas. Adipose SMC.