Airborne particulate matter in an iron mining city: Characterization, cell uptake and cytotoxicity effects of nanoparticles from PM2.5, PM10 and PM20 on human lung cells

• DOI: 10.1016/j.envadv.2021.100125
• Publication Date: 2021-10-12T23:51:11Z
• Authors: Mariana Morozesk; Iara da Costa Souza; Marisa Narciso Fe...; Daniel Cristian F...
• Keywords: Environmental sciences; Emerging contaminants; 13. Climate action; Air quality; 11. Sustainability; GE1-350; Flow cytometry; Nanotoxicology; 01 natural sciences; Risk assessment; 0105 earth and related environmental sciences

Airborne particulate matter (PM) is dispersed into the atmosphere and can internalize in lungs during respiratory process by passive exposure. Itabira city (Minas Gerais, Brazil) a mining with relevant PM emissions derived from iron exploitation, transport, processing. In present study, PM2.5, PM10, PM20 characterization ore its internalization cytotoxicity effects were evaluated MRC-5 human lung cell line. PMs characterized using scanning electron microscopy EDS X-ray analysis and, then, water analyzed Dynamic Light Scattering Nanosight techniques. Flow cytometry was applied to evaluate cellular uptake on cells death induction. The results demonstrate that PM10 mass collected higher than those reported local governmental agencies are mainly constituted hematite quartz. showed presence of Fe, Si, Mg, Al, Zi, Ca, emerging metallic contaminants, such as Ba Ti, environmental samples. Analyzed samples dissociate nanoparticles (< 200 nm) aqueous medium, demonstrating comprises aggregated be internalized cells, leading decrease viability. Thus, reducing exposure may considered potential tool for metal contaminants air diseases. view health problems caused airborne pollution, this study brings new discussions aiming improve safety standards future investigations field worldwide.