Exposure to naphthalene and β-pinene-derived secondary organic aerosol induced divergent changes in transcript levels of BEAS-2B cells

• DOI: 10.1016/j.envint.2022.107366
• Publication Date: 2022-06-21T15:14:46Z
• Authors: Michal Pardo; Svenja Offer; Elena Hartner; Sebastiano Di Buc...; Christoph Bisig; Stefanie Bauer; Jana Pantzke; Elias J. Zimmermann; Xin Cao; Stephanie Binder; Evelyn Kuhn; Anja Huber; Seongho Jeong; Uwe Käfer; Eric Schneider; Arunas Mesceriakovas; Jan Bendl; Ramona Brejcha; Angela Buchholz; Daniela Gat; Thorsten Hohaus; Narges Rastak; Erwin Karg; Gert Jakobi; Markus Kalberer; Tamara Kanashova; Yue Hu; Christoph Ogris; Annalisa Marsico; Fabian Theis; Tali Shalit; Thomas Gröger; Christopher P. Rüger; Sebastian Oeder; Jürgen Orasche; Andreas Paul; Till Ziehm; Zhi-Hui Zhang; Thomas Adam; Olli Sippula; Martin Sklorz; Jürgen Schnelle-K...; Hendryk Czech; Astrid Kiendler-S...; Ralf Zimmermann; Yinon Rudich
• Keywords: Pinene

The health effects of exposure to secondary organic aerosols (SOAs) are still limited. Here, we investigated and compared the toxicities soot particles (SP) coated with β-pinene SOA (SOAβPin-SP) SP naphthalene (SOANap-SP) in a human bronchial epithelial cell line (BEAS-2B) residing at air–liquid interface. SOAβPin-SP mostly contained oxygenated aliphatic compounds from photooxidation, whereas SOANap-SP significant fraction aromatic products under similar conditions. Following exposure, genome-wide transcriptome responses showed an Nrf2 oxidative stress response, particularly for SOANap-SP. Other signaling pathways, such as redox signaling, inflammatory involvement matrix metalloproteinase, were identified have stronger impact following also induced genotoxicity response than that SOAβPin-SP. This study elucidated mechanisms govern toxicity that, SOAs derived typical biogenic precursor, anthropogenic precursor higher toxicological potency, which was accompanied activation varied cellular mechanisms, aryl hydrocarbon receptor. can be attributed difference chemical composition; specifically, naphthalene-derived had cytotoxic potential β-pinene-derived SOA.