Genome-wide alternation and effect of DNA methylation in the impairments of steroidogenesis and spermatogenesis after PM2.5 exposure

• DOI: 10.1016/j.envint.2022.107544
• Publication Date: 2022-09-24T07:51:32Z
• Authors: Zhonghao Zhang; Jiankang Wang; Fuquan Shi; Yingqing Li; Peng Zou; Ying Tang; Chang Liu; Yimeng Wang; Xi Ling; Lei Sun; Cuiqing Liu; Yanshu Zhang; Fei Gao; Qing Chen; Lin Ao; Fei Han; Jinyi Liu; Jia Cao
• Keywords: Epigenomics

The effects of ambient fine particles on male reproductive health have raised widespread concern. particular underlying mechanisms the damage remain largely unclear and demand more research in new directions. Previous has revealed that DNA methylation plays an important role development is also vulnerable to environmental influences. However, there hasn't been enough investigation into involvement PM2.5-induced toxicity. Here, we establish a real-time PM2.5 exposure model could lead testicular dysfunction including spermatogenesis impairment steroid hormone dysfunction. In particular, decrease global level 5-methylcytosine (5mC) indicated possible association with injury induced by exposure. Further genome-wide analysis genomic hypomethylation identified than 1000 differentially methylated regions both CAP UA versus FA, indicating exposure, even low-dose, modulate methylome. Furthermore, integrated methylome transcriptome some key genes networks, which may be involved synthesis hormone. levels especially Cyp11a1 Pax8 raised, their consequent reduced expression impair testosterone sperm production process. Our provides fundamental knowledge as well novel insights harm.