The role of ANXA5 in DBP-induced oxidative stress through ERK/Nrf2 pathway

• DOI: 10.1016/j.etap.2019.103236
• Publication Date: 2019-08-01T20:08:04Z
• Authors: Lei Zhang; Zhiqiang Qin; Ran Li; Shangqian Wang; Wei Wang; Min Tang; Wei Zhang
• Keywords: Malondialdehyde

Di-N-butylphthalate (DBP) have given rise to more and attention due its unique endocrine toxicity male reproductive system. Our previous studies demonstrated antioxidative Nrf2 (nuclear factor erythroid related 2) pathway play a vital role in DBP induced oxidative stress injury. ANXA5 (annexin A5), which is highly expressed testicular Leydig Sertoli cells, was found upregulated after stimulation. Mouse cells were exposed different concentration of for 24 h examine the ROS (Reactive oxygen species), MDA (Malondialdehyde), SOD (superoxide dismutase) level ANXA5, Nrf2, NQO1 (NAD(P)H-quinone oxidoreductase 1), HO-1 (heme oxygenase 1) ERK/P-ERK protein expression by DHE (Dihydroethidium) staining, ELISA (enzyme-linked immunosorbent assay) Western blot respectively. Firstly, injury re-validated. Then, we confirmed change exposure cells. Additionally, overexpressed could activate Nrf2/HO-1/NQO1 antioxidant significantly attenuate DBP-induced stress. Ultimately, increase ERK phosphorylated activated on ERK/Nrf2 be reversed inhibitor. Overall, this study illuminated that defend testicle against through pathway.