Anti-Leishmania activity of essential oil of Myracrodruon urundeuva (Engl.) Fr. All.: Composition, cytotoxity and possible mechanisms of action

• DOI: 10.1016/j.exppara.2017.02.012
• Publication Date: 2017-02-09T09:45:19Z
• Authors: C.E.S. Carvalho; E.P.C. Sobrinho-J...; L.M. Brito; L.A.D. Nicolau; T.P. Carvalho; A.K.S. Moura; K.A.F. Rodrigues; S.M.P. Carneiro; D.D.R. Arcanjo; A.M.G.L. Citó; F.A.A. Carvalho
• Keywords: Amastigote; Axenic; IC50

Myracrodruon urundeuva (Engl.) Fr. All., commonly known as "aroeira-do-sertão", is a medicinal plant from Anacardiaceae family. In this study, the chemical composition of M. essential oil (MuEO) was evaluated by gas chromatography-mass spectrometry (GC-MS), well its anti-Leishmania potential, cytotoxicity, and macrophage activation capability possible antiprotozoal mechanism action were assessed. Fourteen compounds identified, which constituted 94.87% total composition. The most abundant components monoterpenes (80.35%), with β-myrcene (42.46%), α-myrcene (37.23%), caryophyllene (4.28%) major constituents. MuEO inhibited growth promastigotes (IC50 205 ± 13.4 μg mL-1), axenic amastigotes 104.5 11.82 mL-1) decreased percentage infection number per 44.5 4.37 μg⋅mL-1), suggesting significant activity. cytotoxicity assessed MTT test in Balb/c murine macrophages human erythrocytes lysis assay low for these cells observed. CC50 value against 550 29.21 mL-1, while around 20% at highest concentration assessed, HC50 > 800 mL-1. While MuEO-induced activity not mediated increases both lysosomal nitric oxide production macrophages, results suggest antiamastigote associated an immunomodulatory due to increase phagocytic induced MuEO. Thus, presented Leishmania amazonensis, probably modulating erythrocytes.