Metabolomics assisted fingerprint of Hypericum perforatum chemotypes and assessment of their cytotoxic activity
0301 basic medicine
0303 health sciences
Plants, Medicinal
Cell Survival
Plant Extracts
Cytotoxicity
Plant Components, Aerial
Settore BIO/15 - BIOLOGIA FARMACEUTICA
630
3. Good health
Anthocyanins
03 medical and health sciences
Tandem Mass Spectrometry
615
Hypericum perforatum
Humans
Metabolomics
14. Life underwater
Phenolics
Caco-2 Cells
Caco-2 cells
Chromatography, High Pressure Liquid
Hypericum
DOI:
10.1016/j.fct.2018.02.057
Publication Date:
2018-02-27T18:43:11Z
AUTHORS (8)
ABSTRACT
Hypericum perforatum is known as an important medicinal plant, used for the treatment of several diseases, while its pharmacological properties are attributed to the presence of a wide range of secondary metabolites. Due to the great chemotypic variability of Hypericum species in the nature, and the demand for standardized herbal products, a detailed phytochemical investigation was carried out on different parts (herba, leaf, flowers) from wild collected and cultivated populations, using advanced chromatographic tools. Liquid Chromatographic analysis (LC-MS/MS MRM) revealed significant variability in the secondary metabolites content of the examined methanolic extracts. The most common derivatives belong to 9 groups i.e. benzoic acids, phenylpropanoids, coumarins, flavones, flavonols, flavan-3-ols, anthocyanins, phloroglucinols and naphtodianthrones. The main polyphenolic compounds were catechin, epicatechin, quercetin, quercetin 3-O-rhamnoside, quercetin 3-O-glucoside, neochlorogenic acid, proanthocyanidins (A and B series) and cyanidin-3-O-glucoside. In addition, the content of the characteristic compounds hypericin and hyperforin in herba crude extracts ranged between 0.5 and 1.7 mg/g and 0.6-3.3 mg/g respectively. The cytotoxic activity of the crude extracts was assessed at concentrations ranged between 0.01 and 100 μg/mL, on Caco-2 intestinal cancer cell cultures, and a cytotoxic behavior was shown only at the highest concentration of 100 μg/mL.
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