Bisphenol F and bisphenol S promote lipid accumulation and adipogenesis in human adipose-derived stem cells
0301 basic medicine
Adipogenesis
Dose-Response Relationship, Drug
Gene Expression
Cell Differentiation
Mesenchymal Stem Cells
Fatty Acid-Binding Proteins
3. Good health
PPAR gamma
Lipoprotein Lipase
03 medical and health sciences
Phenols
CCAAT-Enhancer-Binding Protein-alpha
Humans
Sulfones
Benzhydryl Compounds
DOI:
10.1016/j.fct.2021.112216
Publication Date:
2021-04-16T05:34:21Z
AUTHORS (7)
ABSTRACT
Bisphenol F (BPF) and bisphenol S (BPS) are increasingly used as substitutes for bisphenol A (BPA), an endocrine disrupting chemical (EDC) with obesogenic activity. We investigated the in vitro effects of BPS and BPF on the adipogenesis of human adipose-derived stem cells (hASCs) exposed to different doses (0.01, 0.1, 1, 10 and 25 μM), stopping the adipogenic process at 7 or 14 days. Intracellular lipid accumulation was quantified by the Oil Red O assay, gene expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAT/enhancer-binding protein (C/EBPα), lipoprotein-lipase (LPL) and fatty acid binding protein 4 (FABP4), by quantitative real-time polymerase chain reaction (qRT-PCR) and protein levels by Western Blot. hASCs with BPF or BPS produced a linear dose-response increase in intracellular lipid accumulation and in gene expression of the adipogenic markers, confirmed by protein levels. Co-treatment ICI 182,780 significantly inhibited BPF- but not BPS-induced lipid accumulation. Given the affinity of bisphenols for diverse nuclear receptors, their obesogenic effects may result from a combination of pathways rather than a single mechanism. Further research is warranted on the manner in which chemicals interfere with adipogenic differentiation. To our best knowledge, this report shows for the first time the obesogenic potential of BPF in hASCs.
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