Kinetics of inclusion body formation studied in intact cells by FT‐IR spectroscopy

• DOI: 10.1016/j.febslet.2005.04.085
• Publication Date: 2005-06-01T13:39:29Z
• Authors: Diletta Ami; Antonino Natalello; Pietro Gatti-Lafr...; Marina Lotti; Silvia Maria Doglia
• Keywords: Inclusion bodies; Amide

The aggregation of a recombinant lipase as inclusion bodies (IBs) was studied directly within intact Escherichia coli cells by FT‐IR microspectroscopy. Through this approach, it possible to monitor in real time the different kinetics IB formation at 37 and 27 °C, excellent agreement with results SDS–PAGE analysis. Furthermore, insights on residual native‐like structure expressed protein – both isolated inside were obtained secondary analysis Amide I band spectra.