Over-expression of sly-miR156a in tomato results in multiple vegetative and reproductive trait alterations and partial phenocopy of the sft mutant

• DOI: 10.1016/j.febslet.2010.12.036
• Publication Date: 2010-12-26T09:08:52Z
• Authors: Xiaohui Zhang; Zhe Zou; Junhong Zhang; Yuyang Zhang; Qinqin Han; Tixu Hu; Xiaoguang Xu; Hui Liu; Hanxia Li; Zhibiao Ye
• Keywords: Phenocopy; Genetically modified tomato

Plant microRNAs (miRNAs) are vital components of the translation control system that regulates plant development and reproduction. The biological function sly-miR156 was investigated by over-expression in tomato plants. Transgenic plants exhibited a drastically altered phenotype, with reduced height, smaller but more numerous leaves, fruit. inflorescence structure over-expressing phenocopied sft mutant. putative targets were identified data base search included six SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-box transcription factor genes. Their expression patterns then determined 35S-miR156a wild type These target genes, as well FLOWERING LOCUS T (FT) ortholog SFT, significantly down-regulated studies reveal novel phenotypes regulated miR156.