TLR‐induced NF‐κB activation regulates NLRP3 expression in murine macrophages

• DOI: 10.1016/j.febslet.2012.02.045
• Publication Date: 2012-03-08T22:54:42Z
• Authors: Yu Qiao; Peng Wang; Jianni Qi; Lei Zhang; Chengjiang Gao
• Keywords: Lipopolysaccharides; 0301 basic medicine; Macrophage; Response Elements; NF-κB; Mice; 03 medical and health sciences; NLRP3; Toll-like receptor; NLR Family, Pyrin Domain-Containing 3 Protein; Animals; RNA, Messenger; Promoter Regions, Genetic; Cells, Cultured; Cell Line, Transformed; Macrophages; Toll-Like Receptors; NF-kappa B; Macrophage Activation; Recombinant Proteins; DNA-Binding Proteins; Mice, Inbred C57BL; Gene Expression Regulation; Macrophages, Peritoneal; Mutant Proteins; Carrier Proteins

NLRP3 inflammasome plays a critical role in the activation of caspase‐1 and maturation IL‐1β. However, specific cis ‐ trans ‐regulatory elements that determine extent expression are not well defined. In this study, we found was induced by TLR agonists murine macrophages NF‐κB dependent manner. Furthermore, corresponding binding sites (nt −1303 to −1292 −1238 −1228) were identified promoter. Finally, EMSA ChIP assays demonstrated LPS‐induced Therefore, out results delineated molecular mechanisms involved TLR‐induced transcriptional regulation NLRP3.