MicroRNA‐145 suppresses mouse granulosa cell proliferation by targeting activin receptor IB

• DOI: 10.1016/j.febslet.2012.06.048
• Publication Date: 2012-07-13T08:58:15Z
• Authors: Guijun Yan; Lianxiao Zhang; Ting Fang; Qun Zhang; Shaogen Wu; Yue Jiang; Haixiang Sun; Yali Hu
• Keywords: Granulosa cell; Proliferation; Smad2 Protein; Mice; 03 medical and health sciences; Animals; Cyclin D2; Humans; RNA, Messenger; Phosphorylation; MicroRNA-145; 3' Untranslated Regions; Cells, Cultured; Smad; Cell Proliferation; Mice, Inbred ICR; 0303 health sciences; Granulosa Cells; Base Sequence; Activin receptor IB; Activins; MicroRNAs; HEK293 Cells; Female; Activin Receptors, Type I; Signal Transduction

MicroRNAs (miRNAs) are a class of 21‐ to 25‐nucleotide non‐coding RNAs, some of which are important gene regulators involved in folliculogenesis. In this study, we used CCK‐8, real‐time PCR and Western blot assays to demonstrate that miR‐145 inhibits mouse granulosa cell (mGC) proliferation. Combined with the results of luciferase reporter assays that studied the 3′‐untranslated region of ACVRIB mRNA, these assays identified ACVRIB as a direct target of miR‐145. The ectopic expression of miR‐145 reduced the levels of both ACVRIB mRNA and protein and also interfered with activin‐induced Smad2 phosphorylation. Altogether, this study revealed that miR‐145 suppresses mGC proliferation by targeting ACVRIB.