Impact of oil contamination and biostimulation on the diversity of indigenous bacterial communities in soil microcosms

• DOI: 10.1016/j.femsec.2004.04.007
• Publication Date: 2004-05-08T07:19:35Z
• Authors: Flavia F. Evans; Alexandre S. Rosado; Gina V. Sebastián; Renata Casella; Pedro L.O.A. Machado; Carola Holmström; Staffan Kjelleberg; Jan D. Elsas; Lucy Seldin
• Keywords: Microcosm; Biostimulation; Soil test

The aim of this study was to analyse the effect oil contamination and biostimulation (soil pH raise, nitrogen, phosphate sulphur addition) on diversity a bacterial community an acidic Cambisol under Atlantic Forest. experiment based enumeration populations hydrocarbon degraders in microcosms through use conventional plating techniques molecular fingerprinting samples directly from environment. PCR followed by denaturing gradient gel electrophoresis (DGGE) used generate microbial fingerprints employing 16S rRNA gene as marker. Biostimulation led increases soil (to 7.0) levels phosphorus K, Ca, Mg. Oil caused increase organic carbon (170-190% higher than control soil). Total counts were stable throughout experiment, while MPN showed biostimulated oil-contaminated samples. Molecular performed with DGGE analysis revealed patterns along 360 days showing little change after 90 days. severe changes due decreases number bands compared 15 addition nutrients soil. Results obtained present indicate that inorganic compounds conjunction has greater impact only.