Gamma delta T cell responses associated with the development of tuberculosis in health care workers

• DOI: 10.1016/j.femsim.2004.09.005
• Publication Date: 2004-10-14T15:19:00Z
• Authors: Diane J. Ordway; Luisa Pinto; Leonor Costa; Marta Martins; Clara Leandro; Miguel Viveiros; Leonard Amaral; Maria J. Arroz; Fernando A. Ventura; Hazel M. Dockrell
• Keywords: Adult; Cytotoxicity, Immunologic; Male; 0301 basic medicine; 0303 health sciences; Infectious Disease Transmission, Patient-to-Professional; Portugal; Health Personnel; Receptors, Antigen, T-Cell, gamma-delta; In Vitro Techniques; Middle Aged; Lymphocyte Activation; 3. Good health; Occupational Diseases; Interferon-gamma; 03 medical and health sciences; T-Lymphocyte Subsets; Humans; Female; Interleukin-4; Lymphocyte Count; Tuberculosis, Pulmonary

This study evaluated T cell immune responses to purified protein derivative (PPD) and Mycobacterium tuberculosis (Mtb) in health care workers who remained free of active (HCWs w/o TB), went on develop TB w/TB), non-health were (Non-HCWs) patients presenting with minimal (Min TB) or advanced (Adv disease. Peripheral blood mononuclear cells (PBMC) stimulated Mtb PPD the expression activation markers CD25+ HLA-DR+, intracellular IL-4 IFN-γ production cytotoxic evaluated. PBMC from HCWs developed showed decreased percentages expressing CD8+CD25+ comparison healthy. increased γδ TCR+ cytotoxicity CD3+γδ disease CD25+CD4+ CD25+CD8+ that associated CD8+ phenotypes, reduced cytotoxicity. Our results suggest show an early compensatory mechanism involving increase lytic which did not prevent TB.