Colorimetric determination of indole-3-carbaldehyde by reaction with carbidopa and formation of aldazine in ethanolic extract of cabbage

• DOI: 10.1016/j.focha.2024.100643
• Publication Date: 2024-02-15T17:06:37Z
• Authors: Pasquale Palladino; Lorenzo Attanasio; Simona Scarano; Ilaria Degano; Maria Minunni
• Keywords: Absorbance; Colored

A colorimetric assay was developed for detection of indole-3-carbaldehyde (I3A), a bioactive compound particularly abundant in vegetables the Brassicaceae family, inducing plant defense responses and ensuring human intestinal barrier integrity. The is based on selective condensation reaction between hydrazine group carbidopa, used as derivatizing agent, aldehyde I3A, generating colored aldazine with characteristic absorbance around 415 nm. As proof concept, among several natural sources here reported quantitative data collection, assessed white heart cabbage ethanolic extract. I3A content determined by LC-MS analysis, which confirmed formation matrix. LOD (10.4 ± 0.3 μM) obtained calibration curve from visible (m = 565 16 M−1, avRSD% 6.5, R2 0.9960) indicates that this simple, safe, low-cost method could be beneficial also current methodologies identification quantification foods.