Molecular characterization and biological effects of a CXCL8 homologue in large yellow croaker (Larimichthys crocea)

• DOI: 10.1016/j.fsi.2015.03.026
• Publication Date: 2015-04-05T21:43:43Z
• Authors: Yinnan Mu; Kunru Wang; Jingqun Ao; Xinhua Chen
• Keywords: Inflammation; 0301 basic medicine; DNA, Complementary; Base Sequence; Chemotaxis; Interleukin-8; Molecular Sequence Data; Sequence Analysis, DNA; Head Kidney; Real-Time Polymerase Chain Reaction; Perciformes; 3. Good health; Fish Diseases; 03 medical and health sciences; Gene Components; Bacterial Vaccines; Escherichia coli; Animals; Cluster Analysis; Electrophoresis, Polyacrylamide Gel; Amino Acid Sequence; Phylogeny; Spleen

CXCL8 also called interleukin-8, is a CXC-type chemokine that plays a key role in promoting inflammation. Three subgroups of CXCL8 homologues have been reported in teleost fish, including CXCL8_L1, CXCL8_L2 and CXCL8_L3. In the present study, we identified a CXCL8 homologue belonging to CXCL8_L1 subgroup (LycCXCL8_L1) in large yellow croaker (Larimichthys crocea) that shares low identity to the previously reported large yellow croaker CXCL8 (LycCXCL8). The full-length cDNA of LycCXCL8_L1 is 716 nucleotides (nt) long and encodes a protein consisting of 99 amino acids (aa) with a putative molecular weight of 11.2 kDa. The deduced LycCXCL8_L1 protein contains a 22-aa signal peptide and a 77-aa mature polypeptide, which possesses an arrangement of four cysteines typical of other known CXC chemokines (C(34), C(36), C(60), and C(77)). Genomic analysis revealed that the LycCXCL8_L1 gene consisted of four exons and three introns and exhibited a similar exon-intron organization to LycCXCL8 and other species CXCL8 genes except for a different intron length. Phylogenetic analysis showed that both LycCXCL8_L1 and LycCXCL8 belong to CXCL8_L1 subgroup. LycCXCL8_L1 mRNA was constitutively expressed in all tissues examined although at different levels. Upon bacterial vaccine induction, LycCXCL8_L1 mRNA expression was rapidly increased in the spleen and head kidney tissues. Recombinant LycCXCL8_L1 and LycCXCL8 proteins produced in Escherichia coli both induced chemotaxis and superoxide production in peripheral blood leucocytes from large yellow croaker. These results indicate that two CXCL8_L1 molecules exist in large yellow croaker and play roles in inflammatory response.