Nondestructive extraction DNA method from bones or teeth, true or false?

• DOI: 10.1016/j.fsigss.2015.09.111
• Publication Date: 2015-10-02T20:36:05Z
• Authors: C. Gomes; S. Palomo-Díez; J. Roig; A.M. López-Parra; C. Baeza-Richer; A. Esparza-Arroyo; J. Gibaja; E. Arroyo-Pardo
• Keywords: 03 medical and health sciences; 0302 clinical medicine; Ancient DNA; Non-destructive analysis; DNA extraction; Critical samples

One of the most challenging points for ancient DNA studies on human remains is that analysing procedures involve partial or total destruction of the samples, which usually are unique and irreplaceable. This is what prevents museums, anthropologists or archaeologists from giving samples for genetic investigation. So that it is interesting to find an analysing method without destroying them. In this study, it was carried out the evaluation of a non-destructive extraction DNA protocol on 4 Neolithic individuals, from Can Gambús (Sabadell, Spain) and 4 pre Bell Beaker period individuals, from Los Cercados (Valladolid, Spain). Along this work, it was discussed the efficiency of the non-destructive protocol, valuing the obtained result, and the evidences state of preservation after the whole procedure.<br/>This work has been possible by the project HAR2009-10105 funded MINECO of the Spanish Government and the BES2010-035322FPI grant, for P-D.S., by G/6401400/8000 research project (Santander-Universidad Complutense de Madrid, Spain) for C.G, and by the HAR2011-23149 research project funded by the MICINN of the Spanish Government for J.G.<br/>Peer Reviewed<br/>