Differential responses of genes and enzymes associated with ROS protective responses in the sugarcane smut fungus

• DOI: 10.1016/j.funbio.2020.09.009
• Publication Date: 2020-10-04T13:51:27Z
• Authors: L.P. Peters; N.S. Teixeira-Silva; A.P. Bini; M.M.L. Silva; N. Moraes; G.S. Crestana; S. Creste; R.A. Azevedo; G. Carvalho; C.B. Monteiro-Vit...
• Keywords: Smut

The fungal pathogen Sporisorium scitamineum causes sugarcane smut disease. We have previously shown that resistant plants induce ROS, coinciding with a delay in colonization. Here, we investigated whether the fungus modifies enzymatic antioxidant system vitro and when colonizing tissues response to ROS. In vitro, exposure ROS did not affect cell integrity, combination of superoxide dismutases (SOD) catalases (CAT) were active. alter expression transcriptional regulator Yap1 effector Pep1. activated distinct enzymes plant tissues. Instead CAT, S. induced glutathione peroxidase (Gpx) only smut-resistant plants. had an earlier both smut-susceptible -resistant plants, no apparent correlation genes sod, cat, gpx, or external redox imbalance. pep1 was potentially These results collectively suggest copes oxidative stress by inducing different mechanisms depending on conditions (in vitro/in planta) intensity Moreover, Pep1 is responsive imposed genotype.