Gene expression signatures affected by ethanol and/or nicotine in normal human normal oral keratinocytes (NHOKs)

• DOI: 10.1016/j.gdata.2014.06.021
• Publication Date: 2014-06-27T23:00:21Z
• Authors: Jeffrey J. Kim; Omar Khalid; Lewei Duan; Reuben Kim; David Elashoff; Yong Kim
• Keywords: 0301 basic medicine; 570; Nicotine; Oncology and Carcinogenesis; 610; QH426-470; 7. Clean energy; Alcohol Use and Health; Substance Misuse; 03 medical and health sciences; Stem Cell Research - Nonembryonic - Human; Tobacco; Data in Brief; Genetics; 2.1 Biological and endogenous factors; Dental/Oral and Craniofacial Disease; 0303 health sciences; Biomedical and Clinical Sciences; Human Genome; Data management and data science; Gene expression signatures; Pharmacology and Pharmaceutical Sciences; Biological Sciences; Stem Cell Research; 3. Good health; Alcoholism; Good Health and Well Being; 13. Climate action; Biochemistry and Cell Biology; Normal human oral keratinocytes; Alcohol

It has been reported that nicotine/alcohol alter epigenetic control and lead to abrogated DNA methylation and histone modifications, which could subsequently perturb transcriptional regulation critically important in cellular transformation. The aim of this study is to determine the molecular mechanisms of nicotine/alcohol-induced epigenetic alterations and their mechanistic roles in transcriptional regulation in human adult stem cells. We hypothesized that nicotine/alcohol induces deregulation of epigenetic machinery and leads to epigenetic alterations, which subsequently affect transcriptional regulation in oral epithelial stem cells. As an initiating step we have profiled transcriptomic alterations induced by combinatory administration of EtOH and nicotine in primary normal human oral keratinocytes. Here we provide detailed experimental methods, analysis and information associated with our data deposited into Gene Expression Omnibus (GEO) under GSE57634. Our data provide comprehensive transcriptomic map describing molecular changes induced by EtOH and nicotine on normal human oral keratinocytes.