Transcriptomic profiling of human embryonic stem cells upon cell cycle manipulation during pluripotent state dissolution

• DOI: 10.1016/j.gdata.2015.08.032
• Publication Date: 2015-08-31T17:31:30Z
• Authors: Kevin Andrew Uy G...; Hongqing Liang
• Keywords: 0301 basic medicine; Embryonic stem cells; 03 medical and health sciences; Differentiation; Data in Brief; Genetics; QH426-470; Cell cycle; Microarray

While distinct cell cycle structures have been known to correlate with pluripotent or differentiated cell states [1], there is no evidence on how the cell cycle machinery directly contributes to human embryonic stem cell (hESC) pluripotency. We established a determinant role of cell cycle machineries on the pluripotent state by demonstrating that the specific perturbation of the S and G2 phases can prevent pluripotent state dissolution (PSD) [2]. Active mechanisms in these phases, such as the DNA damage checkpoint and Cyclin B1, promote the pluripotent state [2]. To understand the mechanisms behind the effect on PSD by these pathways in hESCs, we performed comprehensive gene expression analysis by time-course microarray experiments. From these datasets, we observed expression changes in genes involved in the TGFβ signaling pathway, which has a well-established role in hESC maintenance [3], [4], [5]. The microarray data have been deposited in NCBI's Gene Expression Omnibus (GEO) and can be accessed through GEO Series accession numbers GSE62062 and GSE63215.