Expression of C/EBP and Kr-h1 transcription factors under immune stimulation in the noble crayfish

• DOI: 10.1016/j.gene.2024.148813
• Publication Date: 2024-08-01T00:14:51Z
• Authors: Ljudevit Luka Boš...; Paula Dragičević; Lena Bonassin; Caterina Francesconi; Anita Tarandek; Leonie Schardt; Christelle Rutz; Sandra Hudina; Klaus Schwenk; Odile Lecompte; Kathrin Theissinger
• Keywords: Laminarin

Transcription factors (TFs) have an important role in the regulation of gene expression network. The TFs immune response freshwater crayfish is poorly understood, but leveraging regulatory mechanisms could augment resistance against invasive oomycete pathogen, Aphanomyces astaci. Previous studies indicated that CCAAT/enhancer-binding protein (C/EBP) and putative Krüppel homolog-1 (Kr-h1) might play a stress noble (Astacus astacus). Here, we aimed to further characterise these two products gain better understanding their evolutionary origin, domain organisation patterns across different tissues. Furthermore, conducted stimulation experiment observe potential changes C/EBP Kr-h1 under challenge Our results showed both are closely related other C/EBPs Kr-h1s Malacostraca. Gene analysis revealed present all analysed tissues, with higher gills abdominal muscle. Immune laminarin (mimicking β-1-3-glucan cell wall) activation system, overall increase total haemocyte count (THC) compared untreated control buffered saline (CBS) treatment. On level, up-regulation was detected treated group hepatopancreas heart, while no were observed for gene. indicate early change multiple tissues during suggest its involvement crayfish.