In vivo antiplasmodial activity of hydromethanolic leaf extract and solvent fractions of Maytenus gracilipes (Celastraceae) against Plasmodium berghei in mice

• DOI: 10.1016/j.heliyon.2021.e08457
• Publication Date: 2021-11-24T17:10:06Z
• Authors: Dejen Nureye; Muktar Sano Kedir; Rekik Ashebir Muluye; Workineh Woldesel...; Eyob Tekalign
• Keywords: Celastraceae; Plasmodium berghei

BackgroundThe incidence of resistance among currently available antimalarial drugs, as well the high economic cost malaria, has prompted researchers to look for novel molecules. As a result, current study was proposed evaluate antiplasmodial activity (in vivo) Maytenus gracilipes based on plant's traditional claims.MethodsA cold maceration procedure using 80% methanol solvent employed obtain crude extract from M. leaves. Chloroform, n-butanol, and pure water were used fractionate hydromethanolic extract. Standard procedures followed an acute oral toxicity test. The effects plant at 200, 400, 600 mg/kg doses investigated three rodent malaria models (4-day suppressive, rane's, repository tests). Thirty mice utilized in each experiment (3 treatment 2 control groups, with six mice). Parasitemia, survival time, body weight, temperature, packed cell volume all assess extracts' activity. To compare results between one-way ANOVA Post Hoc Tukey's HSD used.ResultsIn 4-day suppressive investigation, fractions suppressed parasitemia significantly (P < 0.001) compared negative control. had greatest chemosuppressive effect (74.15%) dose. Chloroform suppression fractions; however it less than In Rane's test, produced substantial curative control.ConclusionAccording this study, leaves contain effect. more active chloroform n-butanol fractions, indicating that non-polar medium polar constituents are responsible. Nonetheless, further analysis is required isolate characterize compounds responsible