Evaluation of SARS-CoV-2 rapid antigen diagnostic tests for saliva samples

• DOI: 10.1016/j.heliyon.2022.e08998
• Publication Date: 2022-02-22T06:54:55Z
• Authors: Marie Hagbom; Noelia Carmona-Vi...; Sumit Sharma; Henrik Olsson; Mikael Jämtberg; Åsa Nilsdotter-Au...; Johanna Sjöwall; Johan Nordgren
• Keywords: H1-99; 0301 basic medicine; SARS-CoV-2; Rapid antigen diagnostic test; Saliva; Infectivity; Science (General); SARS-CoV-2; Rapid antigen diagnostic test; Analytical Chemistry; 3. Good health; Social sciences (General); Q1-390; 03 medical and health sciences; Infectivity; Analytisk kemi; Saliva; Research Article

Using saliva samples would facilitate sample collection, diagnostic feasibility, and mass screening of SARS-CoV-2. We tested two rapid antigen (RAD) immunochromatographic tests designed for detection SARS-CoV-2 in saliva: Rapid Response™ COVID-19 Antigen Test Cassette oral fluids DIAGNOS™ Saliva Test. Evaluation limit was performed with purified nucleocapsid protein live virus. Sensitivity specificity were further evaluated reverse transcription quantitative PCR (RT-qPCR) positive negative from hospitalized individuals (n = 39) healthcare workers 20). DIAGNOS showed higher sensitivity than Response both The the test comparable Abbott Panbio™ Ag nasopharyngeal samples. detected nine (50.0%) seven (38.9%), respectively, 18 RT-qPCR All 41) tests. Only one contained infectious virus as determined by cell culture also using RADs. results show that may be an important easy-to-use RAD complement to detect individuals, but validation a larger set is warranted.