Compressive force regulates GSK-3β in osteoclasts contributing to alveolar bone resorption during orthodontic tooth movement in vivo

• DOI: 10.1016/j.heliyon.2022.e10379
• Publication Date: 2022-08-24T03:53:16Z
• Authors: Yan Liu; Ke Wu; Xing Cui; Yelin Mao
• Keywords: Periodontal fiber; Bone remodeling; X-ray microtomography

Orthodontic tooth movement mainly depends on biological and mechanical reactions in the periodontium, such as indispensable reconstruction process of periodontal ligament alveolar bone. To explore whether orthodontic compressive force can induce bone resorption during by regulating GSK-3β/β-catenin pathway.We established models Sprague-Dawley rats. In addition, force-induced that occurred was analyzed HE staining micro-CT. The number distribution osteoclasts were observed TRAP staining. Furthermore, pressure-induced mediated signaling pathway immunohistochemistry.As shown micro-CT results, parameters, mineral density (BMD), volume fraction (BV/TV), trabecular thickness (Tb. Th), significantly decreased under stimulation, contrast with dramatically increased spacing Sp). During movement, side force, which increases expression phosphorylated Ser-GSK-3β activation β-catenin pathway. Additionally, downregulation GSK-3β activity further caused leading to loss. immunohistochemistry results indicated influenced osteoclast formation secretion osteoclast-related cytokines, matrix metallopeptidase 9 (MMP-9) receptor activator nuclear factor-κB ligands (RANKLs), is also related duration force.These inhibitor promote force. contributes Therefore, be a potential target for clinical applications.