Phytochemical composition and bioactivities of Crataegus aronia as antioxidant, antibacterial and antioxidative stress in red blood cells – Is it a window of hope for children with glucose-6-phosphate dehydrogenase deficiency

• DOI: 10.1016/j.heliyon.2022.e11516
• Publication Date: 2022-11-25T16:25:07Z
• Authors: Ghassab M. Al-Maz...; Ahmed H. Al-Mustafa; Sulaiman Mohammed...; Ibrahim Nassir-Allah; Khaled A. Tarawneh; Fuad Al-Rimawi; Mohamad Nasir Moh...; Mohammed Ayed Huneif; Shifaa O. Alshammari; Asim Ali Yaqoob; Fadel Wedian; Mohammed Helmy Fa...
• Keywords: Phytochemical

Crataegus aronia (C. aronia) extracts have been used medicinally since ancient times and are often utilized in traditional Arab medicine. An extensive study has revealed that species antioxidant, antibacterial, anti-inflammatory, hypotensive properties.This work was performed to explore the phytochemical contents of C. extract, as well its antioxidant antibacterial properties, assess lipid peroxidation level an oxidative stress biomarker erythrocytes.Chemical constituents methanolic extract were identified by gas chromatography-mass spectrometry their relative concentrations determined. The activity determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) ferric reducing power (FRAP) assays. effect on concentration malondialdehyde (MDA) erythrocyte hemolysates studied. Also, crude assessed for antimicrobial through agar diffusion microbroth dilution assays.The DPPH IC50 value showed antioxidants equal (14.3 μg/mL) according FRAP assay, range 33.9 μmol-82.86 μmol Fe+2/g dw. exerts a protective against RBCs shows 50% inhibition malonyldialdehyde at 39.48 μg/mL extract. Minimum inhibitory found 800-1000 leave extracts. analysis total phenols, flavonoids, flavonols content 494.071 mg GAE/g 155.251 RE/g 103.2049 extract). contains alkaloids, terpenoids, steroids. Crude more potent inhibiting growth B. subtilis, S. aureus M. luteus with MIC MBC values 800,800 1000 μg/mL, respectively. According GC-MS, 20 compounds identified: dihydro-3-methylene-5-methyl-2-furanone (14.71%), hexanoic acid (6.57%), ethyl 3,5-ditert-butyl-4-hydroxybenzoate (6.4%), N, N-dimethylheptadecan-1-amine (4.91%), methyl 2-oxobutanoate (4.14%), glyceraldehyde (3.98%), 2-methoxy-1-(2-nitroethenyl)-3-phenylmethoxybenzene (3.16%), major constituents.This may open window hope children Glucose-6-phosphate dehydrogenase disorder possible utilization active ingredients minimize both infection which negatively impact disease sequelae.According these vitro experiments, this plant significant amount natural antioxidants, aid protection various stresses. As result, employing components infection, detrimental sequelae, bring disorder.