Peptide derived C. striata albumin as a natural angiotensin-converting enzyme inhibitor

• DOI: 10.1016/j.heliyon.2023.e15958
• Publication Date: 2023-04-30T00:37:01Z
• Authors: Guntur Berlian; Catur Riani; Neng Fisheri Kurn...; Heni Rachmawati
• Keywords: Tricine

As one of the most popular sources for fish albumin, Channa striata has been considered as a promising substitute human albumin. However, scientific information regarding its genomic and proteomic is very limited, making identification rather complicated. In this study, we aimed to isolate, characterize, examine bioactivity protein peptide derivatives C. Fractionation albumin from extract was conducted using Cohn Process yield evaluated. The peptides were further produced by enzymatic hydrolysis. All these proteins studied tricine-SDS PAGE tested in vitro ACE inhibition. Dry weights Fraction-5, where more abundant purer, 3.8 ± 2.1%. Based on analysis, two bands protein, e.g., approximately 10 13 kDa, detected with highest intensity found which might be striata. An increasing trend inhibition fractions observed, ranging 7.09 22.99%. ACEI activity alcalase hydrolysis molecular size <3 kDa (56.65 2.32%, IC50 36.93 μg/mL). This value also statistically significant compared non-hydrolyzed Fraction-5 Parental Fraction, 23.48 3.11% (P < 0.05) 13.02 0.68% 0.01), respectively. Taken together, findings suggest potential peptide-derived natural antihypertensive agents.