Antimicrobial and in silico studies of the triterpenoids of Dichapetalum albidum

• DOI: 10.1016/j.heliyon.2023.e18299
• Publication Date: 2023-07-14T02:07:24Z
• Authors: Mary A. Chama; Godwin A. Dziwornu; Elizabeth Popli; Eduard Mas-Claret; Beverly Egyir; Daniel M. Ayine-Tora; Kofi B-A. Owusu; David G. Reid; Dorcas Osei-Safo; Melinda Duer; Dulcie Mulholland; Andreas Bender
• Keywords: H1-99; 0303 health sciences; Science (General); 3β-acetoxy-2α,19β-dihydroxy-urs-12-en-28-oic acid; 2β, 6β, 21α-trihydroxyfriedelan-3-one; Trypanosoma brucei brucei; 3. Good health; Social sciences (General); Q1-390; 03 medical and health sciences; Dichapetalum albidum; Glucose-6-phosphate dehydrogenase; Leishmania donovani; Research Article

Here we report a new polyhydroxylated triterpene, 2β,6β,21α-trihydroxyfriedelan-3-one (4) isolated from the root and stem bark of Dichapetalum albidum A. Chev (Dichapetalaceae), along with six known triterpenoids (1-3, 5, 6, 8), sitosterol-3β-O-D-glucopyranoside (9), a dipeptide (7), and a tyramine derivative of coumaric acid (10). Friedelan-3-one (2) showed an antimicrobial activity (IC50) of 11.40 μg/mL against Bacillus cereus, while friedelan-3α-ol (1) gave an IC50 of 13.07 μg/mL against Staphylococcus aureus with ampicillin reference standard of 19.52 μg/mL and 0.30 μg/mL respectively. 3β-Acetyl tormentic acid (5) showed an IC50 of 12.50 μg/mL against Trypanosoma brucei brucei and sitosterol-3β-O-d-glucopyranoside (9) showed an IC50 of 5.06 μg/mL against Leishmania donovani with respective reference standards of IC50 5.02 μg/mL for suramin and IC50 0.27 μg/mL for amphotericin B. Molecular docking of the isolated compounds on the enzyme glucose-6-phosphate dehydrogenase (G6PDH) suggested 3β-acetyl tormentic acid (5) and sitosterol-3β-O-D-glucopyranoside (9) as plausible inhibitors of the enzyme in accordance with the experimental biological results observed.