Network pharmacology and in vivo and in vitro experiments to determine the mechanism behind the effects of Jiawei Yanghe decoction via TLR4/Myd88/NF-κB against mastitis

• DOI: 10.1016/j.heliyon.2023.e21219
• Publication Date: 2023-10-21T07:23:12Z
• Authors: Jing Zhao; Liuyan Xu; Lingyan Lv; Liuyi Wang; Xuan Wang; Chen Liang; Chunhui Wang; Yan Qiu; Xiaohua Pei
• Keywords: Decoction; KEGG

In the Qing dynasty, Yanghe decoction was as a therapeutic soup for effectively treating chronic inflammatory disorders. It used clinical use of decoction, adjustment medication variety diseases have effect, including mastitis. Therefore, Jiawei (JWYHD) may be to treat breast diseases.First, LM- and JWYHD-related components were retrieved from database analysis platform. Next, protein-protein interaction networks constructed screen key targets, gene ontology Kyoto encyclopedia genome enrichment analyses performed predict potential biological functions mechanisms JWYHD. Simultaneously, JWYHD samples collected analyzed by UPLC-HRMS. Finally, in vivo vitro experiments conducted construct animal cellular inflammation models mastitis with LPS. Pathological changes mammary tissues detected. Enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, Western blotting determine mRNA protein levels cytokines toll-like receptor 4/myeloid differentiation primary response 88/nuclear factor kappa B signaling pathway elucidate underlying anti-mastitis effects different aspects.In total, 103 compounds detected 691 active ingredients screened network pharmacology, 47 LM-related targets identified. The PPI revealed 5 core targets. KEGG results established NF-κB pathways core. After intervention, low mild damage observed. Furthermore, treatment affected gland TLR4/Myd88/NF-κB considerably reducing respective expression; thus, alleviated LM symptoms.We hypothesized demonstrated anti-inflammatory cytokine regulation via pathway. conclusion, showed its other acute diseases.