Identification of a novel ferroptosis-related gene signature associated with retinal degeneration induced by light damage in mice

• DOI: 10.1016/j.heliyon.2023.e23002
• Publication Date: 2023-11-30T12:18:58Z
• Authors: Xin-Lan Lei; Qiao-Li Yang; Yong-Zhao Wei; Xu Qiu; Hui-Yi Zeng; Ai-Min Yan; Kai Peng; Ying-Lin Li; Feng-Qin Rao; Feng-Hua Chen; Lue Xiang; Kun-Chao Wu
• Keywords: KEGG; Pyroptosis

Neurodegenerative retinal diseases such as retinitis pigmentosa are serious disorders that may cause irreversible visual impairment. Ferroptosis is a novel type of programmed cell death, and the involvement ferroptosis in degeneration still unclear. This study aimed to investigate related genes mice model induced by light damage.A public dataset GSE10528 deriving from Gene Expression Omnibus database was analyzed identify differentially expressed (DEGs). set enrichment analysis between damage control group conducted. The ferroptosis-related (DE-FRGs) were subsequently identified intersecting DEGs with retrieved FerrDb database. Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) further performed using DE-FRGs. A protein-protein interaction (PPI) network constructed hub (HFRGs). microRNAs (miRNAs)-HFRGs, transcription factors (TFs)-HFRGs networks well target drugs potentially interacting HFRGs utilizing bioinformatics algorithms.A total 932 group. Among these, 25 associated ferroptosis. GO KEGG analyses revealed these DE-FRGs mainly enriched apoptotic signaling pathway, response oxidative stress autophagy, ferroptosis, necroptosis cytosolic DNA-sensing pathway. Through PPI analysis, six (Jun, Stat3, Hmox1, Atf3, Hspa5 Ripk1) ultimately identified. All them upregulated retinas, verified GSE146176 dataset. Bioinformatics predicated 116 miRNAs, 23 TFs several potential therapeutic compounds might interact HFRGs.Our provide biomarkers, targets new insights into landscape neurodegenerative diseases.