Transcription factor FOXP4 inversely governs tumor suppressor genes and contributes to thyroid cancer progression

• DOI: 10.1016/j.heliyon.2023.e23875
• Publication Date: 2024-01-07T22:39:20Z
• Authors: Tian Zhou; Ning Ma; Yong-lin Zhang; Xing-hong Chen; Xue Luo; Mai Zhang; Qing-jun Gao; Dai-wei Zhao
• Keywords: Tumor progression; Transcription

ObjectiveIn recent decades, thyroid cancer (TC) has exhibited a rising incidence pattern. Elevated levels of the transcription factor FOXP4 have been strongly linked to progression diverse tumors; nevertheless, its specific role in remains underexplored. The primary objective this study was elucidate functions and associated target gene, FBXW7, context cancer.MethodsFOXP4 FBXW7 expression TC tissues cell lines were assessed through immunohistochemistry RT-qPCR analyses. functional aspects FOXP4, including effects on proliferation, migration capabilities, cycle regulation, epithelial-mesenchymal transition (EMT), investigated. Furthermore, interaction between confirmed using chromatin immunoprecipitation (ChIP) assays. impact FOXP4-mediated cellular phenotypes subsequently examined. Additionally, vivo tumor growth elucidated establishment murine model.ResultsElevated observed papillary carcinoma tissues, patients exhibiting high showed more favorable prognosis. KTC-1 cells displayed concomitant increase decrease expression. overexpression these enhanced EMT. protein promoter confirmed, mitigated upon FBXW7. knockdown led decelerated transplanted tumors increased within tumors.ConclusionThe findings current underscore regulatory establish clear link aberrations manifestation malignant highly aggressive cells.