Ginger volatile oil inhibits the growth of MDA-MB-231 in the bisphenol A environment by altering gut microbial diversity

• DOI: 10.1016/j.heliyon.2024.e24388
• Publication Date: 2024-01-09T17:41:59Z
• Authors: Liming Luo; Yuran Chen; Qiuting Ma; Yun Huang; Lei Xu; Kun Shu; Zhongfa Zhang; Zhiyong Liu
• Keywords: Bacterial growth

ObjectiveTo examine the impact of ginger volatile oil (GVO) on growth MDA-MB-231 breast cancer cells in presence bisphenol A (BPA) by modulating diversity gut microbiota. Methods: were injected subcutaneously into right armpit female BALB/c Nude (nu/nu) mice to create a triple negative model. Thirty nude randomly divided 5 groups: control group (distilled water every day), BPA PEG-400+ DMSO + cyclodextrin GVO (0.25 mL/kg) group, (0.5 (1 6 each group; The drug was given gavage once day for 4 weeks. At end experiment, changes tumor mass and volume observed compared groups tumor-bearing mice. High-throughput sequencing (16S rRNA) used detect microflora group. Results: weight decreased low, medium high dose GVO. Among them, difference between high-dose reached significant level (P < 0.05). species abundance flora following treatment, but increased after combined treatment with In ratio Firmicutes(F) Bacteroidea(B) respectively 0.10:0.79 at phylum level, while further (0.04:0.88). After feeding GVO, number Firmicutes Bacteroidea increased, F/B Lactobacillus alistipes increased. predominant functions are cell membrane biogenesis, carbohydrate transport metabolism. This is followed amino acid metabolism, transcription function. administration, Gram-positive bacteria (G+) had an increasing trend Gram-negative (G−)ratio decreasing trend. Conclusion: