Identification of differentially expressed miRNAs in plasma exosomes from patients with early-onset pre-eclampsia using next generation sequencing

• DOI: 10.1016/j.heliyon.2024.e24543
• Publication Date: 2024-01-26T13:28:20Z
• Authors: Wuqian Wang; Weihua Zhang; Luan Chen; Xiaojia Wu; Jianmei Gu; Fan Yang; Bo Wang; Shengying Qin; Ping Tang
• Keywords: Identification; Exosome

Pre-eclampsia (PE), a major cause of perinatal morbidity and mortality, accounts for up to 14 % mortality maternal 18 fetal or infant mortalities. However, the pathogenesis process PE remains unclear. The aim this study was identify differentially expressed microRNAs (miRNAs) in peripheral blood exosomes early-onset patients versus healthy pregnant women using high-throughput sequencing, find candidate miRNAs as molecular markers. Methods: Peripheral samples were collected from five preeclamptic women. Exosomal sequenced Illumina HiSeq4000 sequencing platform. target gene prediction, biological function enrichment, signaling pathway prediction with significant differences carried out Starbase database software, Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) databases, respectively. Our results showed 65 significantly compared control group, 17 up-regulated 48 down-regulated (P < 0.05). A total 2231 genes predicted all miRNAs. Biological functions enriched by these mainly associated Ras protein signal transduction, GTPase-mediated transduction regulation, histone modification, β-transforming growth factor regulatory process. Key pathways included TGF-β pathway, PI3K-Akt MAPK tumor necrosis EGFR tyrosine kinase inhibition pathways. QPCR validation 40 independent 10 miRNAs, identified three confirmed second population. MIR7151 most its downstream gene, KCNQ10T1, software. There miRNA expression profiles between normal women, suggesting that may contribute pathophysiology regulating various