Sendai virus is robust and consistent in delivering genes into human pancreatic cancer cells

• DOI: 10.1016/j.heliyon.2024.e27221
• Publication Date: 2024-02-28T17:33:15Z
• Authors: Dmytro Grygoryev; Taelor Ekstrom; Elise Manalo; Jason M. Link; Amani Alshaikh; Dove Keith; Brittany L. Allen...; Brett Sheppard; Terry Morgan; Abdenour Soufi; Rosalie C. Sears; Jungsun Kim
• Keywords: Sendai virus

BackgroundPancreatic ductal adenocarcinoma (PDAC) is a highly intratumorally heterogeneous disease that includes several subtypes and plastic. Effective gene delivery to all PDAC cells essential for modulating expression identifying potential gene-based therapeutic targets in PDAC. Most current systems pancreatic are optimized islet or acinar cells. Lentiviral vectors the main PDAC, but their transduction efficiencies vary depending on cell type, especially poor classical subtype of from both primary tumors lines.MethodsWe systemically compare glycoprotein G vesicular stomatitis virus (VSV-G)-pseudotyped lentiviral Sendai viral human normal cells.ResultsWe find vector gives most robust efficiency regardless type. Therefore, we propose using transduce ectopic genes into