Evaluation the frequencies of HLA alleles in moderate and severe COVID-19 patients in Iran: A molecular HLA typing study

• DOI: 10.1016/j.heliyon.2024.e28528
• Publication Date: 2024-03-26T02:54:04Z
• Authors: Farhad Abolnezhadian; Sara Iranparast; Mojtaba Shohan; Zahra Shokati Esh...; Mahtab Hamed; Maryam Seyedtabib; Roohangiz Nashibi; Mohammad-Ali Assa...; Seyed Ali Mard; Ali Akbar Shayesteh; Niloofar Neisi; Manoochehr Makvandi; Seyed Mohammad Alavi; Gholamreza Shariati
• Keywords: HLA-DRB1

BackgroundSevere acute respiratory syndrome coronavirus 2 was first reported in December 2019 and it has spread globally ever since. The HLA system is crucial directing anti-viral immunity recent studies are investigating the possible involvement of genes on severity immune inflammation different phases COVID-19.MethodsIn this cross-sectional study, peripheral blood-extracted genomic DNAs 109 COVID-19 patients 70 healthy controls were genotyped for alleles HLA-A, HLA-B, HLA-DRB1 loci using sequence-specific primer PCR method.ResultsThe results indicated that frequencies HLA-DRB1*11:01 HLA-DRB1*04:03 significantly higher severe rather than moderates (p: <0.001 0.004, respectively). Also, observed HLA-DRB1*04:01 more frequent moderate (p:0.002). In addition, HLA-B*07:35, HLA-DRB1*07:01 showed compared with 0.031 0.003 Inversely, due to HLA-B*51:01 (p:0.027), HLA-DRB1*11:05 (p:0.003), HLA-DRB1*13:05 (p:0.022), HLA-DRB1*14:01 (p:0.006) individuals patients, they may be associated resistance.ConclusionThe show that, based population differences, type related different, which should clarified by designing large-scale order develop HLA-based treatments vaccines.