Chemical, antioxidant, and antifungal analysis of oregano and thyme essential oils from Ecuador: Effect of thyme against Lasiodiplodia theobromae and its application in banana rot

• DOI: 10.1016/j.heliyon.2024.e31443
• Publication Date: 2024-05-18T04:30:22Z
• Authors: Glenda Pilozo; Mirian Villavicen...; Ivan Chóez-Guaranda; Damon Vera Murillo; Cinthya Duarte Pa...; Christofer Tomalá...; Maria Maldonado-E...; Omar Ruiz-Barzola; Fabián León-Tamariz; Patricia Manzano
• Keywords: Thymol; Carvacrol; Lasiodiplodia theobromae; Trolox

The objective of this study was to evaluate the antioxidant capacity by spectrophotometric methods, in vitro and vivo antifungal effect against Lasiodiplodia theobromae constitution essential oils (EO) oregano thyme comparison with their commercial counterparts. results showed EOs extracted (T-EO), (CT-EO), (O-EO) (CO-EO), demonstrated profiles a radical neutralizing potential (DPPH•) IC50: 1.11± 0.019; 1.08 ± 0.05; 40.56 0.227 0.69 0.004 mg/mL, respectively. They also revealed ferric ion reducing (FRAP) 93.05 0.52; 97.72 0.42; 21.85 0.57 117.24 0.64 mg Eq Trolox/g. A reduction β-carotene degradation 65.71 0.04; 51.97 0.66; 43.58 1.56 57.46 %. total phenol content (Folin-Ciocalteu) 132.97 0.77; 141.89 2.56; 152.04 0.10 25.66 0.40 EGA/g. Chemical characterization performed gas chromatography mass spectrometry (GC-MS) that respective major components samples were thymol (T-EO: 45.78%), (CT-EO: 43.57%), alloaromadendrene (O-EO: 25.17%) carvacrol (CO-EO: 62.06%). Regarding activity, it evident at level, both had MIC 250 ppm while EO 500 ppm; In studies application behavior similar synthetic fungicide, slowing down rot bananas under storage conditions. Finally, partial least squares discriminant analysis (PLS-DA) heat maps suggest p-cymene, carvacrol, linalool, eucalyptol, 4-terpineol, (z)-β-terpineol, alkanhol, caryophyllene, β-myrcene, D-limonene, α-terpinene, α-terpineol, D-α-pinene, camphene, caryophyllene oxide, δ-cadinene, terpinolene as relevant biomarkers associated assessed bioactive properties demonstrating for development botanical biofungicide.