Dihydroartemisinin inhibits HNSCC invasion and migration by controlling miR-195-5p expression

• DOI: 10.1016/j.heliyon.2024.e32522
• Publication Date: 2024-06-06T12:35:55Z
• Authors: Xiaolu Wu; Congwen Yang; Zhongwan Li; Ping Lv; Xiang An; Xiaohe Peng; You Li; Xiaojun Jiang; Xuemei Mao; Donghong Chen; Lifeng Jia; Wei Yuan
• Keywords: Artemisia annua; Immunofluorescence; Gentamicin protection assay

ObjectivesDihydroartemisinin (DHA), an artemisinin derivative extracted from the traditional Chinese medicinal herb Artemisia annua, has potential to suppress head and neck squamous cell carcinoma (HNSCC) progression. However, mechanisms underlying these effects remain unclear. Therefore, we aimed examine of DHA on tumor invasion migration.MethodsHuman HNSCC lines CAL-27 FaDu were exposed varying concentrations (0, 5, 20, 80 μM) for 24 h. Cell proliferation, invasion, migration assessed using CCK8, transwell, wound-healing assays, respectively. Quantitative real-time PCR, western blotting, immunofluorescence used assess expression levels target genes proteins.ResultsDHA suppressed cells. Additionally, miR-195-5p This study revealed significant differences in TENM2 between clinical samples multiple public databases. treatment overexpression significantly reduced cells, which suggested that enhanced inhibitory effect TENM2.ConclusionsThis provides first evidence inhibits by regulating miR-195-5p/TENM2 axis suggesting it as a potentially effective strategy HNSCC.