The mechanism of bile acid metabolism regulating lipid metabolism and inflammatory response in T2DM through the gut-liver axis

• DOI: 10.1016/j.heliyon.2024.e35421
• Publication Date: 2024-08-12T22:04:32Z
• Authors: Yan Wang; Bohan Lv; Nannan Liu; Siyu Tao; Jinfang Dou; Jun Li; Ruxue Deng; Xiuyan Yang; Guangjian Jiang
• Keywords: Roseburia; Carbohydrate Metabolism; CYP8B1; Dysbiosis; Metabolic pathway; CYP27A1

AimsThe main objective of this study was to analyze the changes intestinal microflora and how bile acid metabolic pathways affect lipid metabolism in T2DM through gut-liver axis.MethodsFirstly, 16S rRNA sequencing, metabolomics transcriptomic sequencing were performed on plasma feces clinical subjects determine flora its metabolites. Finally, mice model verified vivo.ResultsT2DM patients have significant disorders. The differential fecal metabolites mainly enriched primary biosynthesis cholesterol patients. After verification, gut microbiota (including up-regulated bacteria associated with BA metabolism, such as lactobacillus bifidobacterial, down-regulated capable producing SCFAs Faecalibacterium, Bacteroides, Romboutsia Roseburia); that result impairment barrier function protein expression blood, intestine liver FGFR4↑, TRPM5↑ CYP27A1↓, which are related homeostasis, TLR6↑, MYD88↑ NF-κB↑, inflammatory response). These aspects together contribute development further disorders glucolipid systemic inflammation patients.ConclusionsChanges may response axis mediated by acids.