Genetic structure of populations of Salvia ceratophylloides endemic to southern Calabria (southern Italy)

• DOI: 10.1016/j.heliyon.2024.e35875
• Publication Date: 2024-08-08T17:03:11Z
• Authors: Valentina Lucia A...; Marta Cavallini; Antonino Di Iorio; Gianluca Lombardo; Giorgio Binelli; Agostino Sorgonà; Carmelo Maria Mus...; Giovanni Spampinato
• Keywords: H1-99; 0301 basic medicine; Science (General); Populations structure; Plant conservation; Endangered species; Social sciences (General); Q1-390; 03 medical and health sciences; SSRs; Genetic variability; Endemic species; Research Article

Assessing the degree of genetic diversity and differentiation of rare or endangered endemic species is essential to evaluate the conservation status of populations and successively implement appropriate conservation strategies. We investigated the population structure of Salvia ceratophylloides Ard., a scapose hemicryptophyte endemic to Calabria (southern Italy), both to answer questions about its genetic structure and to determine whether the actual population size has undergone significant demographic changes in the near past. The data obtained from the census showed that the populations are characterised by a greater number of adult individuals than juveniles and are on declining. The genetic analysis carried out on 99 individuals from four populations of the species under study, shows a mean expected heterozygosity value of 0.50 and an overall differentiation value of 0.083. The population structure shows that the four studied populations are distinct genetic units, genetically linked to four different ancestral gene pools. Bayesian analysis based on ABC models indicates that the present populations underwent a significant reduction in size in the past. This corresponds to the demographic decline at the end of the 19th century, which according to the literature, was due to the strong anthropic pressure (agriculture, grazing, fire and plantations) of Reggio Calabria suburbs. We can therefore conclude that populations are not affected by inbreeding and low genetic diversity and that there is no immediate danger of genetic erosion, and that the problems associated with population decline, past and present, are exclusively due to anthropogenic causes.