Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato

• DOI: 10.1016/j.hpj.2022.12.006
• Publication Date: 2022-12-21T01:29:00Z
• Authors: Ying Wang; Wenxian Gai; Liangdan Yuan; Lele Shang; Fangman Li; Zhao Gong; Pingfei Ge; Yaru Wang; Jinbao Tao; Xingyu Zhang; Haiqiang Dong; Yuyang Zhang
• Keywords: WRKY protein domain; Gene cluster; Transcription

High temperature stress is one of the major environmental factors that affect growth and development plants. Although WRKY transcription play a critical role in responses, there are few studies on regulation heat by factors, especially tomato. Here, we identified group I factor, SlWRKY3, involved thermotolerance First, SlWRKY3 was induced upregulated under stress. Accordingly, overexpression led to an increase, whereas knock-out resulted decreased tolerance Overexpression accumulated less reactive oxygen species (ROS), more ROS This indicated positively regulates In addition, activated expression range abiotic stress-responsive genes scavenging, such as SlGRXS1 gene cluster. Further analysis showed can bind promoters cluster activate their expression. Collectively, these results imply positive regulator through direct binding activating scavenging.