Genome-wide identification of the MATE gene family and functional characterization of PbrMATE9 related to anthocyanin in pear

• DOI: 10.1016/j.hpj.2023.01.006
• Publication Date: 2023-01-19T20:45:21Z
• Authors: Jiawen Sheng; Xuening Chen; Bobo Song; Hainan Liu; Jiaming Li; Runze Wang; Jun Wu
• Keywords: Anthocyanin; 0301 basic medicine; 03 medical and health sciences; Coloration; Pear; Plant culture; PbrMATE9; RNA-seq; MATE gene family; SB1-1110

Plant multidrug and toxic compound extrusion (MATE) genes play an important role in the process of detoxification, plant morphogenesis, anthocyanin accumulation. However, whether MATE gene family functions pear peel coloration is still unknown. To evaluate identify members which are involving accumulation pear. In this study, 85 were identified reference genome 'Dangshansuli' through genome-wide identification. Based on structure phylogenetic tree analysis, was divided into five subfamilies. RNA sequencing quantitative real-time polymerase chain reaction (qRT-PCR) indicated that expression patterns PbrMATEs tissue-specific. 28.24% (24) PbrMATE expressed fruits, 44.71% (38) leaves. Additionally, we found levels PbrMATE9, PbrMATE26, PbrMATE50, PbrMATE69 debagged fruits with red significantly higher than those bagged without peel, according to our bagging/debagging treatment 'Mantianhong'. The pattern PbrMATE9 consistent variation trend content, suggesting it might response light exposure. Subcellular localization showed a membrane protein. More strikingly, transient overexpression promoted pear, structural (PbrCHI, PbrANS, PbrDFR, PbrUFGT) biosynthesis pathway also increased significantly. Through co-expression network transcription factors identified, such as WRKY, COL, GATA, BBX, be involved regulation PbrMATE9. study has enriched genetic resources improved understanding