Establishment of somatic embryogenesis regeneration system and transcriptome analysis of early somatic embryogenesis in litchi

• DOI: 10.1016/j.hpj.2023.12.005
• Publication Date: 2024-04-19T02:20:38Z
• Authors: Yaqi Qin; Bo Zhang; Shiqian Wang; Wuyan Guo; Zhike Zhang; Yonghua Qin; Jietang Zhao; Guibing Hu
• Keywords: Plant culture; Somatic embryogenesis; Litchi chinensis; Transcriptome; LcBBM2; SB1-1110

Litchi chinensis Sonn. is an important economic fruit tree in tropical and subtropical regions. Regrettably, the efficiency of plant regeneration via somatic embryogenesis litchi typically low due to poor conversion embryos plants. The purpose this study was establish a system from immature explants 'Heiye' cultivar litchi. Our results demonstrated that MS medium supplemented with 2.0 mg · L-1 2,4-D optimal for callus induction. For embryo (SE) induction, containing 0.5 g activated charcoal (AC) most effective, while use zeatin (ZT) thidiazuron (TDZ) resulted abnormal embryos. rooting rate 2.15% 17.5%, respectively, were achieved using AC. Furthermore, transcriptome analysis performed on embryogenic (EC), globular (GE), heart (HE) explore molecular mechanisms early embryogenesis. 2 587 common DEGs between EC_vs_GE EC_vs_HE identified, expression patterns these separated into twelve major clusters. GO annotation KEGG pathway revealed implicated hormone signal transduction, auxin-activated signaling pathway, other biological processes. Additionally, differentially transcription factors function LcBBM2 which specifically highly expressed during verified. Overexpression tomato promotes shoot formation. Therefore, can provide theoretical basis technical support genetic breeding improvement