Frequency, characterization, and prognostic analysis of PIK3CA gene mutations in Chinese esophageal squamous cell carcinoma

• DOI: 10.1016/j.humpath.2013.09.011
• Publication Date: 2013-10-04T00:00:50Z
• Authors: Jun Hou; Dongxian Jiang; Jingchuan Zhang; Paul R. Gavine; Songtao Xu; Yalan Liu; Chen Xu; Jie Huang; Yunshan Tan; Hao Wang; Yachao Lu; Li Zheng; Yingyong Hou; Lijie Tan
• Keywords: Male; 0301 basic medicine; China; Esophageal Neoplasms; Class I Phosphatidylinositol 3-Kinases; Prognosis; Survival Analysis; Disease-Free Survival; 3. Good health; Phosphatidylinositol 3-Kinases; 03 medical and health sciences; Lymphatic Metastasis; Mutation; Carcinoma, Squamous Cell; Humans; Female; Esophageal Squamous Cell Carcinoma

PIK3CA gene mutations are found in numerous cancers but correlate differently with prognosis. Although the frequency of PIK3CA gene mutation in esophageal squamous cell carcinoma (ESCC) has been previously studied, a prognostic analysis has not been reported. Ninety-six surgically resected ESCC tissues were collected from Chinese patients and DNA was extracted. Gene mutations in PIK3CA (exons 9 and 20), EGFR (exons 18, 19, 20 and 21), KRAS (exons 2 and 3), and BRAF (exons 11 and 15) were screened using mutant-enriched liquid chip technology. PIK3CA gene mutations were identified in 12 of 96 ESCC cases (12.5%). No mutations were identified in EGFR, KRAS or BRAF genes in this study. Correlations between clinicopathological features and PIK3CA mutation status were analyzed and finally, patient survival information was used to determine the prognostic significance of PIK3CA mutation. Interestingly, the frequency of PIK3CA mutation was higher in female ESCC patients (31.3%, 5/16) than in males (8.8%, 7/80), and higher in patients with non-lymph node metastasis (19.6%, 10/51, P = .013) than in patients with lymph node metastasis (4.4%, 2/45, P = .025). Furthermore, patients with PIK3CA-mutated tumors showed a trend towards favorable overall survival (P = .085) but not disease-free survival (P = .238), suggesting that PIK3CA gene status may be a favorable predictive marker in ESCC patients.