Inhibition of bacterial growth on marble stone of 18th century by treatment of nanoencapsulated essential oils

• DOI: 10.1016/j.ibiod.2020.104909
• Publication Date: 2020-01-22T10:51:40Z
• Authors: Ida Romano; Giuseppe Granata; Annarita Poli; Ilaria Finore; Edoardo Napoli; Corrada Geraci
• Keywords: Nanocapsules; Biocide; Bacterial growth; Biocompatible material

Controlling of cultural heritage biodeterioration is a serious problem in the world. Chemical biocides, used to kill unwanted microorganism, can represent a risk for human health and environment, and interfere with the restoration material. Natural biocides could represent a valid alternative to conventional ones. In this study we report the use of nanocapsules suspensions (NCs) loaded with Origanum vulgare and Thymus capitatus essential oils (EOs) to contrast the development of bacterial growth of two microrganisms (Escherichia coli and Kokuria rhizophila) on the marble stone from 18th century church altar. No structural change was observed on the stone after treatment with the aqueous suspension containing nanoparticles as evidenced by SEM-EDX analysis. The nanostructurate systems (EO-NCs) were able to inhibit the bacterial grow on the stone pretreated with bacterial inoculum as showed by agar discs contact test. Nanocapsules, based on biodegradable and biocompatible polymer (poly(?-caprolactone)), loaded with thyme EO are more efficient than nanocapsules loaded with oregano EO. The obtained results evidenced the potential of these natural biocides in the treatment of biodeteriorated cultural heritage.