A reference gene set for chemosensory receptor genes of Manduca sexta

• DOI: 10.1016/j.ibmb.2015.09.007
• Publication Date: 2015-09-12T15:16:36Z
• Authors: Christopher Koenig; Ariana Hirsh; Sascha Bucks; Christian Klinner; Heiko Vogel; Aditi Shukla; Jennifer H. Mansf...; Brian Morton; Bill S. Hansson; Ewald Grosse-Wilde
• Keywords: Arthropod Antennae; Male; 0303 health sciences; Gene Expression Profiling; Genome, Insect; Receptors, Ionotropic Glutamate; Receptors, Odorant; Receptors, Pheromone; Smell; 03 medical and health sciences; Manduca; Multigene Family; Animals; Female; Transcriptome; Phylogeny

The order of Lepidoptera has historically been crucial for chemosensory research, with many important advances coming from the analysis of species like Bombyx mori or the tobacco hornworm, Manduca sexta. Specifically M. sexta has long been a major model species in the field, especially regarding the importance of olfaction in an ecological context, mainly the interaction with its host plants. In recent years transcriptomic data has led to the discovery of members of all major chemosensory receptor families in the species, but the data was fragmentary and incomplete. Here we present the analysis of the newly available high-quality genome data for the species, supplemented by additional transcriptome data to generate a high quality reference gene set for the three major chemosensory receptor gene families, the gustatory (GR), olfactory (OR) and antennal ionotropic receptors (IR). Coupled with gene expression analysis our approach allows association of specific receptor types and behaviors, like pheromone and host detection. The dataset will provide valuable support for future analysis of these essential chemosensory modalities in this species and in Lepidoptera in general.