Real-time PCR assays for the detection of tissue and body fluid specific mRNAs

• DOI: 10.1016/j.ics.2005.10.064
• Publication Date: 2006-04-21T21:37:53Z
• Authors: R. Fang; C.F. Manohar; C. Shulse; M. Brevnov; A. Wong; O.V. Petrauskene; P. Brzoska; M.R. Furtado
• Keywords: Body fluid; Identification

Identification of tissue parts and body fluids is frequently required in crime scene investigations. We analysed expression data from public databases and internal studies to identify 110 highly expressed mRNAs as potential tissue specific markers. Further, we identified specific assays targeting mRNA in body fluids like saliva, semen, vaginal secretions and blood. The capability to pre-amplify small amounts of RNA enabling testing for the presence of multiple mRNA species was demonstrated. D 2005 Elsevier B.V. All rights reserved.